The most important group of biting dipterans are mosquitoes. Mosquitoes have a long, slender body, needle-shaped mouth parts and legs. The wings sometimes have distinguishable scale patterns. Some mosquito species bite in the evening, morning and night, while some mosquitoes bite during the day. (Rozendaal, 1997). Many tropical diseases such as filariasis, malaria, dengue, dengu hemorrhagic fever, Japanese encephalitis, yellow fever etc. they are transmitted by different species of mosquitoes. Approximately 100 species out of 3000 mosquito species act as vectors of human diseases (Rozendaal, 1997; Rahuman et al., 2008; Arivoli and Tennyson, 2012). Lymphatic filariasis is transmitted by several mosquito species, namely Culex, Anopheles, and Aedes mosquito species. The infection commonly occurs in childhood and causes hidden damage to the lymphatic system. Elephantiasis of lymphatic edema and swelling of the scrotum occurs later in life and permanent disability of the patient is found. Patients suffer social, mental and financial losses that contribute to stigma and poverty, including physically. Worldwide, 947 million people in 54 countries suffer from lymphatic filariasis and require chemotherapy to prevent this neglected disease. Say no to plagiarism. Get a tailor-made essay on "Why Violent Video Games Shouldn't Be Banned"? Get an original essay In 2000, over 120 million people were infected, of whom approximately 40 million were disfigured and incapacitated by this terrible disease. Globally, there were 25 million people affected by lymphatic filariasis with hydrocele and over 15 million people with lymphedema. Manifestations of chronic diseases are found in approximately 36 million people. Lymphatic filariasis is caused by roundworms (family Filariodidea). There are 3 types of filariasis worms: Wuchereria bancrofti (which causes 90% of filariasis), Brugia malayi (which causes most of the remaining cases), and Brugia fears (which also causes the diseases). The immune system of an infected person is destroyed by the adult worms. Adult worms produce millions of microfilariae that circulate in the human bloodstream. When mosquitoes ingest blood, microfilaria enter the mosquito's body. The microfilaria develop into infective larvae inside the mosquito. When infected mosquitoes bite people, the mature microfilaria enter the lymphatic vessels of the human body and develop into adult worms and then the transmission cycle continues (WHO, 2017). In 2000, WHO launched the Global Program to Eliminate Lymphatic Filariasis (GPELF). In 2012, WHO reconfirmed the target date for eliminating the neglected disease, filariasis, by 2020. WHO recommended mass drug administration (MDA) to stop the spread of the infection for elimination. of lymphatic filariasis (LF). MDAs consist of a combined dose of 2 drugs administered annually as preventive chemotherapy measures and these drugs are 400 mg of albendazole and ivermectin (150-200 mcg/kg) or with diethylcarbamazine citrate (DEC) (6 mg/kg). These medicines have a limited effect on adult Wuchereria bancrofti, but are very effective on microfilaria. From 2000 to 2015, 6.2 billion treatments were provided to more than 820 million people to prevent the spread of the disease in many places. Cambodia, Niue, Sri Lanka, Vanuatu, Maldives and Cook Islands have achieved the goal of eliminating filariasis. The WHO recommendation strategy for the elimination of lymphatic filariasis has been successfully implemented by thirteen other countries. In 54 countries chemotherapy is still necessary to eradicate the disease. (WHO,2017). In India, Wuchereria bancrofti and Brugia malayi are two causative agents of lymphatic filariasis. But 95% of filariasis is caused by the Wuchereria bancrofti parasite and 5% by the Brugia malayi parasite. Brugia malayi is widespread in the states of Tamil Nadu, Andhra Pradesh, Kerala, Odisha, West Bengal, Madhya Pradesh and Assam. India alone accounts for 40% of the total global disease burden (Michael et al., 1996). Seventeen states and six Union Territories in India have been identified as endemic with around 553 million people exposed to the risk of infection and among them, around 146 million live in urban areas and the rest in rural areas. In India, it is estimated that approximately 31 million people are carriers of microfilaria and over 23 million people suffer from manifestations of filariasis (WHO, 2005). The highest endemicity was found in Bihar (over 17%), followed by Kerala (15.7%) and Uttar Pradesh (14.6%). The 10% endemic has been found in the states of Andhra Pradesh and Tamil Nadu. The lowest endemicity was found in Goa (less than 1%) followed by Lakshadweep (1.8%), Madhya Pradesh (above 3%) and Assam (around 5%). B. malayi is prevalent in the states of Kerala, Tamil Nadu, Andhra Pradesh, Orissa, Madhya Pradesh, Assam and West Bengal (Regu et al., 2005; National Vector Borne Disease Control Program, 2010). Mosquito control is essential for the eradication of mosquito-borne diseases, improving environmental quality and public health. The primary tool and technique for mosquito control is the application of synthetic insecticides. In the past, control measures for mosquito vectors were based on the frequent and indiscriminate use of synthetic chemical-based insecticides, namely carbamates, organochlorines, organophosphates and pyrethroids (Liu et al., 2006). Indiscriminate use of insecticides has led to increased selection pressure on mosquitoes leading to the development of insecticide-resistant varieties among mosquitoes (Raghvendra, 2002; Kumar et al., 2012). A variety of insecticide resistance has been developed in Culex quinquefasciatus against cyhalothrin, fenthion and cypermethrin, tenerphos. Insecticide residues enter the ecosystem through the food chain. The negative effects of insecticide use have therefore necessitated the need for research and development on indigenous, biodegradable and environmentally safe methods for the control of mosquito species (Tikar et al., 2008). Therefore, in recent years, the use of many of the older synthetic insecticides for mosquito control has been limited due to the high cost of pesticides, concern for environmental sustainability, lack of new pesticides, adverse health effects humans and other animals, the increased rate of biological magnification, their non-biodegradable nature, and the development of insecticide-resistant varieties (Brown, 1986; Russell, 2009). Consequently, the Environmental Protection Act of 1969 created a set of rules and regulations to verify the application of chemical control agents in nature (Bhatt and Khanal, 2009). Since ancient times, even before the discovery of synthetic insecticides, many herbal products have been used as natural insecticides. Botanicals such as pyrethrum, chrysanthemum, derris, quassia, nicotine, turpentine, hellebore, azadirachtin, etc. they were used as botanical insecticides in the pre-DDT era (Shaalan et al., 2005). One of the approaches to control mosquito-borne diseases is to interrupt disease transmission by killing, using repellents, or using larvicides for large-scale larval mortality in vector breeding centers. Currently, mosquito control strategies have failed due to the development of more and more varietiesresistant to insecticides (Brown, 1986; Georghion and Lagunestjida, 1991; WHO, 1992; Kelm et al., 1997; Su and Mulla, 1998; Gericke et al., 2002). ; Haegreaves et al., 2003). Various organophosphates (Temiphos and Fenthion) and insect growth regulators (Diflubenzuron and Methoprene) are widely used as larvicides for mosquito control (Rozendaal, 1997). These synthetic pesticides have caused the development of resistant varieties of mosquitoes, ecosystem imbalance, and damage to mammals including humans (Georghiou and Langunes-tejeda et al., 1991). The best alternative way to prevent these problems could be the use of insecticidal botanicals, which are degradable in nature and their source is renewable. Plants evolved alongside insects to produce secondary metabolites for their chemical defense mechanisms. More than 2000 plant species have been identified that produce secondary metabolites with value for biological pest control programs and among these, 344 species with significant activity against mosquitoes have been identified (Remia and Logaswamy, 2009). Larvicidal, pupicidal, adulticidal, oviposition deterrent, smoke toxicity or repellent activity have been identified by plant species belonging to different families such as Asteraceae, Labiatae, Cladophoraceae, Miliaceae, Rutaceae, Solanaceae, Oocystaceae, Caricaceae etc. (Shaalan et al., 2005; Rawani et al., 2012; Even before the discovery of synthetic organic insecticides more than 50 years ago, some herbal products, namely anabasine and lupinine, the Russian grass alkaloids , Anabasis aphylla; nicotine from Nicotiana tabacum leaves; rotenone from Derris eliptica and pyrethrum from Chrysanthemum cinererifolium flowers have been used as herbal insecticides for vector control due to the low cost, biodegradable nature, and environment etc. (Campbell et al., 1993; Zubairi et al., 2004; Hartzell and Willcoxon, 1941) Hartzell and Willcoxon (1941) worked with 150 plant species to observe toxicity against mosquitoes and many of them demonstrated the efficacy. Sarita et al. In 2012 they collected 15 plant species from local areas of New Delhi, India and obtained extracts of different solvents from different plant parts. Each extract was screened to explore its efficacy as a mosquito larvicidal agent against the first four stages of the dengue vector, Aedes a Egypti. 10 plants showed larvicidal potential and further evaluation of the larvicidal efficacy of the extracts established that the hexane leaf extract of Lantana camara is the most effective extract having a significant LC50 value of 30.71 ppm while the Phyllanthus emblica fruit extract was found to be less effective having a larvicidal value LC50 value of 298.93 ppm. Ranaweera, 1996 screening of some Sri Lankan plants to observe the larvicidal activity of mosquitoes and out of 53 plant species tested, 18 plants showed larvicidal activity against Culex quinquefasciatus mosquito species. Mondal et al., 2016 experimented on 32 plant species and of these 8 plants showed larvicidal activity using crude extracts against Culex quinquefasciatus mosquito species. Amer and Mehlhorn (2006) demonstrated the repellency of forty-one preparations based on essential oils against Aedes, Anopheles and Culex mosquitoes. Jantal et al. (2003) evaluated 17 methanol extracts and 9 essential oil preparations of Malaysian plants for their larvicidal activities against Aedes a Egypti. Roark, 1947 described approximately 1200 species of plants that possessed insecticidal potential. Sukumar et al., 1991 listed and discussed 344 plant species that showed only mosquito-killing activity. Ghosh et al., 2012 examined a large number of plants that have anti-mosquito efficacy.The insecticidal activity of plant extracts varies depending on the plant species, the mosquito species, the geographical varieties, the plant part, the extraction methodology adopted and the polarity of the solvents used during the extraction. A wide range of plants from shrubs, grasses and large trees have been used for extraction to obtain toxic phytochemicals to kill mosquitoes. Phytochemicals are extracted from whole plants such as small herbs or from different parts, i.e. leaves, fruits, barks, stems, roots etc. Of larger plants or trees. In all cases, the most toxic substances are used for mosquito control (Shaalan et al., 2005). Secondary metabolites, present in plants, act as a defense system against parasite/insect attacks. The presence of secondary metabolites of the plant, namely steroids, alkaloids, terpenoids, phenols, flavanoids, etc. They act as moulting hormones, imitators of juvenile hormones, antifeedants, oviposition deterrents, repellents, growth inhibitors, antimoulting hormones, attractants, etc. and these properties of secondary metabolites responsible for the biological activity of plant extracts against target parasites (Champagne et al., 1986). The Annonaceae family consists of trees, shrubs or rarely lianus. The family includes 108 (accepted) genera and approximately 2400 known species. Plants of the Annonaceae family grow mainly in the Tropics; nearly 900 species grow in the neotropics; 450 species in Afrotropical; a few species in the temperate regions and the other species in the Indomalayan. Annona reticulata Linn. belongs to the Annonaceae family. It is also known as Custard Apple, Sugar Apple, Sweet Apple, Ramphalam, Sitaphala, Sarifa etc. His homeland is South America and the West Indies. It is widely distributed in India, Bangladesh, Pakistan and Thailand (Kaleem, 2006; Satyanarayana, 2013). It is a small deciduous and semi-evergreen tree with a height of between 8 and 10 meters, with a spreading or rounded crown and a trunk measuring approximately 25-35 cm in diameter. The leaves are glabrous, straight and pointed at the apex and wrinkled in some varieties. The leaves are narrow-lanceolate, alternate and deciduous in nature, evident veining and the leaves measure approximately 10-20 cm long and 2-5 cm wide. The flowers are yellow-green in colour, fragrant in pendulous, thin clusters, with 3 narrow and fleshy external petals 2-3 cm long which never open completely. The fruits vary in structure, such as spherical, heart-shaped, irregular or oblong and measure approximately 8-16 cm in diameter. The ripe fruit is brown or yellowish, with red reflections and more or less reticulated, depending on the variety and the skin is thin. The pulp is thick, a little grainy and creamy white in color. There is a central pointed fibrous core that is attached to the thick stalk, extending from the middle of the fruit which is a specialty of the fruit (Mahdeem, 1998; Duke, 1993). In Ayurveda A. reticulata is used for the treatment of cancer, dysentery, epilepsy, heart problems, worm infestations, constipation, hemorrhage and also has antifertile, antitumor and aborfactive properties. (Kaleem, 2006). Ripe fruits are a good tonic and sedative. It increases blood circulation, increases muscle strength, reduces burning sensation, decreases the tendency to biliousness and vomiting (Morton, 1987). The leaves of A. reticulata are used in the treatment of colic. The decoction of the leaves is used to treat malaria and syphilis (Duke, 1993). The root bark is used against toothache, and the roots of the plant are used in the form of a decoction prepared for fever (www.worldagroforestrycentre.org). Some people in the Philippines use the heated leaves to apply them on the abdomen to get,.