The main goal of this article is to see whether Kitb/kitlgb is required for HSCs in zebrafish model, whether osm is a new cytokine important for HSCs in zebrafish model, whether osmr and kitb signal are required sequentially for HSC specification and whether osmr and kitb synergize to expand HSCs in the caudal hematopoietic tissue region. These are important because understanding the molecular pathways that control the specification and expansion of hematopoietic stem cells is necessary for the development and execution of regenerative drugs. Say no to plagiarism. Get a tailor-made essay on "Why Violent Video Games Shouldn't Be Banned"? Get an original essayHa: si:ch73-47f2. 1 is the zebrafish ortholog of mammalian OSM, the gene encoding the osm protein Ho: si:ch73-47f2. 1 is not the zebrafish ortholog of mammalian OSM4. osm expression was not detectable by in situ hybridization (IHS), so cECs (caudal embryonic cells) were isolated by FACS (fluorescence-activated cell sorting). This is a method of sorting a mixture of cells one at a time into separate containers using electrical charges based on the light scattering and fluorescent properties of each cell. After the cells were isolated, they were amplified using qPCR (quantitative polymerase chain reaction). This is done by denaturing a target strand of DNA, annealing it with another strand to isolate the area of ​​interest, and elongating it as in normal transcription. The difference is that a fluorescent label is added to quantify the amplified DNA as it amplifies. The data represented in the figures are averages of biological triplicates, meaning that the entire procedure was performed 3 separate times to control for biological variation. eGFP+ on the x-axis is an enhanced green fluorescent protein used in qPCR to detect the presence of the target protein, in this case, osm on the zebrafish flk1 gene. On the y-axis, a fold change simply represents increased protein expression. A change of 0-50 plotted on the y-axis represents a 2-fold change. This means that the gene expresses twice as much in 100 as in 50. In the graph, it is evident that there was more than double as much osm expression in the caudal hematopoietic tissue (CHT) or, in the tail, compared to the rest of the embryo. Based on the graph results, it is evident that osm is expressed in high volumes in the CHT of the zebrafish model. The expression of osm in zebrafish, as in mammals, indicates a role in hematopoiesis (blood cell formation), also as in mammals, supporting the hypothesis that si:ch73-47f2. 1 is the ortholog of the mammalian OSM gene encoding the osm protein involved in hematopoiesis, thus failing to reject the null hypothesis. Please note: this is just a sample. Get a custom paper from our expert writers now. Customize EssayThe information shows how OSM expands HSCs within the caudal hematopoietic tissue. It shows that there was a significant increase in the number of HSCs in the CHT of embryos injected with osm mRNA. Osmr is the receptor that binds to osm to activate it. This helps demonstrate that osm and its receptor osmr play a role in the expansion of HSCs in the CHT. One of the main questions of this article was to see if there is an expansion of HSCs (hematopoietic stem cells) in the caudal hematopoietic tissue (CHT) caused by a synergy between osmr and kitb. This information helps narrow down OSMR as a factor in HSC expansion in the CHT. The role of kitb should now be analyzed to further help answer this question.